WORKING PROTOCOLS

Treatment of mycoplasma infected cell cultures

Plasmocin treatment requires little hands-on manipulation and is completed in only two weeks. Typically, Plasmocin is used at 25 µg/ml which represents a 1:1000 dilution of the 25 mg/ml stock solution. Working concentration of Plasmocin ranges from 12.5 to 37.5 µg/ml.

1. Split an actively dividing culture of cells into medium containing 12-25µg/ml of Plasmocin.
2. Remove and replace with fresh Plasmocin containing medium every 3-4 days for 2 weeks.
3. For maintenance of a Mycoplasma free culture, continue the use of Plasmocin at a concentration of 5µg/ml.

Note: If mycoplasma elimination is not completed after a two week treatment, you may continue the treatment for an additional week and/or increase the concentration to 37.5µg/ml.

Maintenance or prophylactic use against mycoplasma infections

To prevent Mycoplasma and related cell wall-less bacteria contaminations of cell cultures that have been previously tested to be contamination-free, use Plasmocin prevention at a concentration of 5µg/ml that represents a 1:500 dilution of the 2.5 mg/ml stock solution.

TIPS & TRICKS

Plasmocin will not harm frozen cell lines when thawed. Thus it is a good idea to routinely use Plasmocin when reviving cell lines from a frozen stock. This is a good way to ensure a clean start and avoid contaminating cell culture labs because of dubious frozen sample.

Read also the Frequently Asked Questions ( FAQ )